Aqueous humor dynamics and tonometry are crucial for understanding intraocular pressure (IOP) regulation and diagnosing ocular conditions like glaucoma. Aqueous humor, produced by the ciliary body, maintains IOP through its flow and drainage, primarily via the trabecular meshwork. Tonometry measures IOP, with techniques like Goldmann applanation tonometry being essential in glaucoma diagnosis and management. For UG students, mastering these concepts is vital for early detection of glaucoma, monitoring IOP changes in various conditions, and understanding the role of aqueous humor in ocular health. Additionally, it highlights the clinical significance of IOP in managing ocular hypertension and post-surgical care.